Fyn-SH3_5WT_S25C
(Plasmid #118824)

• Purpose: Bacterial expression of the SH3 domain of human Fyn
• Depositing Lab: Nicolas Fawzi
• Publication: Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-19531. doi: 10.1074/jbc.RA118.005120. Epub 2018 Nov 5.

Backbone

• Vector backbone: Unknown
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FYN
• Species: H. sapiens (human)
• Mutation: S25C; See depositor comments below.
• Entrez Gene: FYN (a.k.a. SLK, SYN, p59-FYN)
• Tag / Fusion Protein:
  • 6-His-TEV tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The insert has an V88E variant compared to the NCBI reference [NP_002028.1] but should function as described in the associated publication.