hnRNPA2_190-303
(Plasmid #118822)

• Purpose: Bacterial expression of hnRNPA2 truncation (residues 190 to 302).
• Depositing Lab: Nicolas Fawzi
• Publication: Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-19531. doi: 10.1074/jbc.RA118.005120. Epub 2018 Nov 5.

Backbone

• Vector backbone: pJ411
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hnRNPA2
• Species: H. sapiens (human)
• Mutation: contains amino acids 190-302
• Entrez Gene: HNRNPA2B1 (a.k.a. HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, IBMPFD2, OPMD2, RNPA2, SNRPB1)
• Tags / Fusion Proteins:
  • His tag (N terminal on insert)
  • TEV (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry