pDM_T7_HisLacZomega
(Plasmid
#118815)
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PurposeT7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118815 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET15b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelacZ_
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Alt namebeta-galactosidase omega subunit
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Insert Size (bp)3212
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Tag
/ Fusion Protein
- histag (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CCTGCCACCATACCCACGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDM_T7_HisLacZomega was a gift from Alexander Green (Addgene plasmid # 118815 ; http://n2t.net/addgene:118815 ; RRID:Addgene_118815) -
For your References section:
Low-cost detection of norovirus using paper-based cell-free systems and synbody-based viral enrichment. Ma D, Shen L, Wu K, Diehnelt CW, Green AA. Synth Biol (Oxf). 2018;3(1):ysy018. doi: 10.1093/synbio/ysy018. Epub 2018 Sep 19. 10.1093/synbio/ysy018 PubMed 30370338
