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pHBS1397 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G]
(Plasmid #118812)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118812 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBI
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TARDBP mutant
  • Species
    H. sapiens (human)
  • Mutation
    W385G in CTD of full-length TDP43
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Category 3: TDP43 splicing reporters

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBS1397 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G] was a gift from Rajat Rohatgi (Addgene plasmid # 118812 ; http://n2t.net/addgene:118812 ; RRID:Addgene_118812)

  • For your References section:

    Phase separation-deficient TDP43 remains functional in splicing. Schmidt HB, Barreau A, Rohatgi R. Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2. 10.1038/s41467-019-12740-2 PubMed 31653829
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