-
PurposeVPS13C internally tagged with mClover3 at residue 1914.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 118760 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP-N1
- Backbone size w/o insert (bp) 4015
- Total vector size (bp) 15964
-
Modifications to backboneExcised EGFP
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsGrow at 32 degrees Celsius increasing time of growth by 50%
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameVPS13C
-
SpeciesH. sapiens (human)
-
Insert Size (bp)11949
-
Entrez GeneVPS13C (a.k.a. BLTP5C, PARK23)
-
Tag
/ Fusion Protein
- mClover3 inserted at position aa 1914
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
VPS13C^mClover3 was a gift from Pietro De Camilli (Addgene plasmid # 118760 ; http://n2t.net/addgene:118760 ; RRID:Addgene_118760) -
For your References section:
VPS13A and VPS13C are lipid transport proteins differentially localized at ER contact sites. Kumar N, Leonzino M, Hancock-Cerutti W, Horenkamp FA, Li P, Lees JA, Wheeler H, Reinisch KM, De Camilli P. J Cell Biol. 2018 Oct 1;217(10):3625-3639. doi: 10.1083/jcb.201807019. Epub 2018 Aug 9. 10.1083/jcb.201807019 PubMed 30093493