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VPS13A^Halo
(Plasmid #118759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118759 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 14000
  • Modifications to backbone
    Excised EGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow at 32 degrees Celsius increasing time of growth by 50%
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VPS13A
  • Alt name
    Chorein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    10000
  • Entrez Gene
    VPS13A (a.k.a. BLTP5A, CHAC, CHOREIN)
  • Tag / Fusion Protein
    • Halo tag inserted at position 1372

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VPS13A^Halo was a gift from Pietro De Camilli (Addgene plasmid # 118759 ; http://n2t.net/addgene:118759 ; RRID:Addgene_118759)

  • For your References section:

    VPS13A and VPS13C are lipid transport proteins differentially localized at ER contact sites. Kumar N, Leonzino M, Hancock-Cerutti W, Horenkamp FA, Li P, Lees JA, Wheeler H, Reinisch KM, De Camilli P. J Cell Biol. 2018 Oct 1;217(10):3625-3639. doi: 10.1083/jcb.201807019. Epub 2018 Aug 9. 10.1083/jcb.201807019 PubMed 30093493
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