pMTMVi-N
(Plasmid
#118756)
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PurposeIntermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118756 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC18
- Total vector size (bp) 2376
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Vector typeIntermediate to build tobacco mosaic virus recombinant clones
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTobacco mosaic virus cDNA fragment with a cloning polylinker
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SpeciesTobacco mosaic virus
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMTMVi-N was a gift from Jose-Antonio Daros (Addgene plasmid # 118756 ; http://n2t.net/addgene:118756 ; RRID:Addgene_118756) -
For your References section:
Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Shi X, Cordero T, Garrigues S, Marcos JF, Daros JA, Coca M. Plant Biotechnol J. 2018 Nov 22. doi: 10.1111/pbi.13038. 10.1111/pbi.13038 PubMed 30521145
