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Addgene

GFP-C-CARMIL2b
(Plasmid #118741)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118741 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGFP1-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARMIL2
  • Species
    H. sapiens (human)
  • Entrez Gene
    CARMIL2 (a.k.a. CARMIL2b, IMD58, LRRC16C, RLTPR)
  • Promoter CMV
  • Tag / Fusion Protein
    • AcGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (unknown if destroyed)
  • 3′ cloning site Hind III (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-C-CARMIL2b was a gift from John Cooper (Addgene plasmid # 118741 ; http://n2t.net/addgene:118741 ; RRID:Addgene_118741)

  • For your References section:

    CARMIL2 is a novel molecular connection between vimentin and actin essential for cell migration and invadopodia formation. Lanier MH, Kim T, Cooper JA. Mol Biol Cell. 2015 Dec 15;26(25):4577-88. doi: 10.1091/mbc.E15-08-0552. Epub 2015 Oct 14. 10.1091/mbc.E15-08-0552 PubMed 26466680
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