Skip to main content
Addgene

p3XFlag-CARMIL2
(Plasmid #118740)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118740 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    p3XFlag-CMV10
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARMIL2
  • Species
    H. sapiens (human)
  • Entrez Gene
    CARMIL2 (a.k.a. CARMIL2b, IMD58, LRRC16C, RLTPR)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3x Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (unknown if destroyed)
  • 3′ cloning site Hind III (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3XFlag-CARMIL2 was a gift from John Cooper (Addgene plasmid # 118740 ; http://n2t.net/addgene:118740 ; RRID:Addgene_118740)

  • For your References section:

    CARMIL2 is a novel molecular connection between vimentin and actin essential for cell migration and invadopodia formation. Lanier MH, Kim T, Cooper JA. Mol Biol Cell. 2015 Dec 15;26(25):4577-88. doi: 10.1091/mbc.E15-08-0552. Epub 2015 Oct 14. 10.1091/mbc.E15-08-0552 PubMed 26466680
Related items:
Commonly requested with: