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pBOB-CARMIL2 BH domain-GFP
(Plasmid #118736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118736 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSC-SP-PW-GFP
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARMIL2 BH domain
  • Species
    H. sapiens (human)
  • Entrez Gene
    CARMIL2 (a.k.a. CARMIL2b, IMD58, LRRC16C, RLTPR)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (unknown if destroyed)
  • 3′ cloning site Age I (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This GFP fusion localizes well to plasma membranes, making it a good marker for fluorescence microscopy experiments.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBOB-CARMIL2 BH domain-GFP was a gift from John Cooper (Addgene plasmid # 118736 ; http://n2t.net/addgene:118736 ; RRID:Addgene_118736)

  • For your References section:

    Cell Migration and Invadopodia Formation Require a Membrane-binding Domain of CARMIL2. Lanier MH, McConnell P, Cooper JA. J Biol Chem. 2016 Jan 15;291(3):1076-91. doi: 10.1074/jbc.M115.676882. Epub 2015 Nov 17. 10.1074/jbc.M115.676882 PubMed 26578515
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