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Addgene

pBOB-VE-cadherin-tdTomato
(Plasmid #118733)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118733 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSC-SP-PW-tdTomato
  • Modifications to backbone
    tdTomato cDNA was replaced with GFP in pCSC-SP-PW-GFP vector with Age I and Not I
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cadherin 5 (CD144)
  • Species
    H. sapiens (human)
  • Mutation
    I503T (Common SNP-see comments)
  • Entrez Gene
    CDH5 (a.k.a. 7B4, CD144)
  • Promoter CMV
  • Tag / Fusion Protein
    • tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (unknown if destroyed)
  • 3′ cloning site Age I (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

tdTomato cDNA was replaced with GFP in pCSC-SP-PW-GFP vector with Age I and Not I

I503T is a common SNP that does not affect function. Depositor confirms the expressed protein localizes to cadherin-based cell-cell junctions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBOB-VE-cadherin-tdTomato was a gift from John Cooper (Addgene plasmid # 118733 ; http://n2t.net/addgene:118733 ; RRID:Addgene_118733)
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