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pBOB-VE-cadherin-GFP
(Plasmid #118732)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118732 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSC-SP-PW-GFP
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cadherin 5 (CD144)
  • Species
    H. sapiens (human)
  • Mutation
    I503T (Common SNP-see comments)
  • Entrez Gene
    CDH5 (a.k.a. 7B4, CD144)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (unknown if destroyed)
  • 3′ cloning site Age I (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

I503T is a common SNP that does not affect function. Depositor confirms the expressed protein localizes to cadherin-based cell-cell junctions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBOB-VE-cadherin-GFP was a gift from John Cooper (Addgene plasmid # 118732 ; http://n2t.net/addgene:118732 ; RRID:Addgene_118732)

  • For your References section:

    Septins regulate junctional integrity of endothelial monolayers. Kim J, Cooper JA. Mol Biol Cell. 2018 Jul 15;29(13):1693-1703. doi: 10.1091/mbc.E18-02-0136. Epub 2018 May 17. 10.1091/mbc.E18-02-0136 PubMed 29771630
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