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pLenti CMV TRE3G Neo GFP-Progerin
(Plasmid #118710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti CMV TRE3G Neomycin
  • Backbone manufacturer
    Eric Campeau
  • Backbone size w/o insert (bp) 8013
  • Total vector size (bp) 10645
  • Vector type
    Mammalian Expression, Lentiviral ; TET-ON inducible
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Construct may be grown in DH5A cells, and or 37 degrees, though STBL3 and 30 degrees are recommended to reduce chance of recombination events.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP-Progerin
  • Alt name
    Progerin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2632
  • GenBank ID
    ENSG00000160789
  • Entrez Gene
    LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
  • Promoter CMV TRE3G (TET-ON)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer tgaccagtttactccctatcagtg
  • 3′ sequencing primer CATAGCGTAAAAGGAGCAACA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To generate mammalian inducible cell lines co-infect with lentivirus made from:
pLenti CMV rtTA3 Hygro (w785-1)( Addgene Plasmid #26730)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMV TRE3G Neo GFP-Progerin was a gift from Tom Misteli (Addgene plasmid # 118710 ; http://n2t.net/addgene:118710 ; RRID:Addgene_118710)

  • For your References section:

    A high-content imaging-based screening pipeline for the systematic identification of anti-progeroid compounds. Kubben N, Brimacombe KR, Donegan M, Li Z, Misteli T. Methods. 2015 Sep 1. pii: S1046-2023(15)30070-0. doi: 10.1016/j.ymeth.2015.08.024. 10.1016/j.ymeth.2015.08.024 PubMed 26341717
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