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pcDNA1-Tastin
(Plasmid #11871)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11871 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA1
  • Backbone size w/o insert (bp) 4033
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Use HB10/P3 strain, MC1061/P3 causes mutations. Select transformant in LB plus tetracyclin (10 ug/ml), at 37C. (supF)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tastin
  • Alt name
    TROAP
  • Species
    H. sapiens (human)
  • GenBank ID
    U04810.2
  • Entrez Gene
    TROAP (a.k.a. TASTIN)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unk (unknown if destroyed)
  • 3′ cloning site Unk (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA1-Tastin was a gift from Michiko Fukuda (Addgene plasmid # 11871 ; http://n2t.net/addgene:11871 ; RRID:Addgene_11871)

  • For your References section:

    Trophinin and tastin, a novel cell adhesion molecule complex with potential involvement in embryo implantation. Fukuda MN, Sato T, Nakayama J, Klier G, Mikami M, Aoki D, Nozawa S. Genes Dev. 1995 May 15. 9(10):1199-210. 10.1101/gad.9.10.1199 PubMed 7758945
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