pER8 cHA-FG1
(Plasmid #118689)

• Purpose: Plant inducible expression
• Depositing Lab: Kristiina Himanen
• Publication: Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi: 10.3389/fpls.2017.00416. eCollection 2017.

Backbone

• Vector backbone: pER8 cHA
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: AT3G07200
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 549
• Entrez Gene: AT3G07200 (a.k.a. AT3G07200)
• Promoter: LexA
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

28 C in GV3101 (pMP90) strain

Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:

Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5


Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
   


Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9