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Addgene

pDEST 544-PHL2
(Plasmid #118672)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118672 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST 544
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PHL2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    897
  • Entrez Gene
    AT3G24120 (a.k.a. AT3G24120)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-NusA (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:

Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5


Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
   


Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST 544-PHL2 was a gift from Kristiina Himanen (Addgene plasmid # 118672 ; http://n2t.net/addgene:118672 ; RRID:Addgene_118672)

  • For your References section:

    Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin E3 Ligases in Arabidopsis. Pavicic M, Mouhu K, Wang F, Bilicka M, Chovancek E, Himanen K. Front Plant Sci. 2017 Mar 28;8:416. doi: 10.3389/fpls.2017.00416. eCollection 2017. 10.3389/fpls.2017.00416 PubMed 28400782
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