SITS-EGFP-ccdb
(Plasmid
#118628)
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PurposeMultisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZ
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118628 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneI-SceI
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameccdb
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SITS-EGFP-ccdb was a gift from Rob Parton (Addgene plasmid # 118628 ; http://n2t.net/addgene:118628 ; RRID:Addgene_118628) -
For your References section:
In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation. Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim YW, Parton RG. Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w. 10.1038/s41467-020-17486-w PubMed 32709891