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Addgene

pENTR-PIP-FUCCI
(Plasmid #118621)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118621 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    ThermoFisher Scientific
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 4560
  • Modifications to backbone
    none
  • Vector type
    Gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIP-FUCCI
  • Species
    Synthetic
  • Insert Size (bp)
    1992
  • Tags / Fusion Proteins
    • mVenus (C terminal on insert)
    • mCherry (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-PIP-FUCCI was a gift from Jean Cook (Addgene plasmid # 118621 ; http://n2t.net/addgene:118621 ; RRID:Addgene_118621)

  • For your References section:

    Accurate delineation of cell cycle phase transitions in living cells with PIP-FUCCI. Grant GD, Kedziora KM, Limas JC, Cook JG, Purvis JE. Cell Cycle. 2018;17(21-22):2496-2516. doi: 10.1080/15384101.2018.1547001. 10.1080/15384101.2018.1547001 PubMed 30421640
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