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pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA
(Plasmid #118617)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118617 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti PGK Puro DEST (w529-2)
  • Backbone manufacturer
    Eric Campeau
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 9624
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TK-NLS-mTurq2-PCNA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1677
  • Entrez Gene
    PCNA (a.k.a. ATLD2)
  • Promoter hPGK
  • Tag / Fusion Protein
    • mTurq2

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA was a gift from Jean Cook (Addgene plasmid # 118617 ; http://n2t.net/addgene:118617 ; RRID:Addgene_118617)

  • For your References section:

    Accurate delineation of cell cycle phase transitions in living cells with PIP-FUCCI. Grant GD, Kedziora KM, Limas JC, Cook JG, Purvis JE. Cell Cycle. 2018;17(21-22):2496-2516. doi: 10.1080/15384101.2018.1547001. 10.1080/15384101.2018.1547001 PubMed 30421640
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