pLenti-PGK-Neo-PIP-FUCCI Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pLenti-PGK-Neo-PIP-FUCCI with the following enzymes: PvuI-HF
We expect and 3 bands: 7.3, 1.8, 1 kb. The unexpected 4th band and ~4.6kb appears to be contamination from the original entry vector.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb Ladder and a digestion of the plasmid pLenti-PGK-Neo-PIP-FUCCI with the following enzymes: PvuI-HF
Expected bands:
For PvuI-HF, 3 bands (~7.3 + 1.8 + 1 kb)
Results:
We observe the expected bands for this digest.
