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pMCh1807_pBS_cdc42E7-boxB
(Plasmid #118612)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118612 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS
  • Backbone size w/o insert (bp) 3311
  • Total vector size (bp) 4724
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cdc42E7 3'UTR
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1409
  • GenBank ID
    NM_009861 NM_009861
  • Entrez Gene
    Cdc42
  • Promoter T3
  • Tag / Fusion Protein
    • boxB (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer caggaaacagctatgac
  • 3′ sequencing primer gatccactagttctaga
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Addgene NGS unable to fully resolve the poly-A tail length. Please refer to the depositor's sequence for this section of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCh1807_pBS_cdc42E7-boxB was a gift from Marina Chekulaeva (Addgene plasmid # 118612 ; http://n2t.net/addgene:118612 ; RRID:Addgene_118612)

  • For your References section:

    Alternative 3' UTRs direct localization of functionally diverse protein isoforms in neuronal compartments. Ciolli Mattioli C, Rom A, Franke V, Imami K, Arrey G, Terne M, Woehler A, Akalin A, Ulitsky I, Chekulaeva M. Nucleic Acids Res. 2018 Dec 22. pii: 5258023. doi: 10.1093/nar/gky1270. 10.1093/nar/gky1270 PubMed 30590745
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