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pMRE-Tn5-154
(Plasmid #118536)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118536 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAG408
  • Vector type
    Bacterial Expression ; miniTn5 delivery vector
  • Selectable markers
    Chloramphenicol/ Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli S17-1 lambda pir
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mOrange2
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mOrange2-pBAD

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRE-Tn5-154 was a gift from Mitja Remus-Emsermann (Addgene plasmid # 118536 ; http://n2t.net/addgene:118536 ; RRID:Addgene_118536)

  • For your References section:

    Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria. Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, Dobson RCJ, Remus DM, Remus-Emsermann MNP. Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. eCollection 2018. 10.3389/fmicb.2018.03052 PubMed 30631309
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