pMRE130
(Plasmid
#118484)
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Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteria
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118484 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFru97
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Vector typeBacterial Expression
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Selectable markersChloramphenicol
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli S17-1 lambda pir
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTagBFP2
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made bymTagBFP2-pBAD
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRE130 was a gift from Mitja Remus-Emsermann (Addgene plasmid # 118484 ; http://n2t.net/addgene:118484 ; RRID:Addgene_118484) -
For your References section:
Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria. Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, Dobson RCJ, Remus DM, Remus-Emsermann MNP. Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. eCollection 2018. 10.3389/fmicb.2018.03052 PubMed 30631309
