pMRE130
(Plasmid #118484)

• Purpose: broad-host range plasmid to constitutively express fluorescent protein genes in bacteria
• Depositing Lab: Mitja Remus-Emsermann
• Publication: Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. eCollection 2018.

Backbone

• Vector backbone: pFru97
• Vector type: Bacterial Expression
• Selectable markers: Chloramphenicol

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. coli S17-1 lambda pir
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TagBFP2
• Species: Synthetic

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: mTagBFP2-pBAD
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.