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Addgene

pLEX307 GFP-Kif26b-C
(Plasmid #118483)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118483 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEX_307
  • Backbone manufacturer
    Addgene #41392 (provided by David Root)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse Kif26b-C
  • Alt name
    Kif26b WRD domain (Wnt5a regulated degradation domain)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1140
  • Mutation
    Encodes GFP fused to amino acid 1735 to 2112 of mouse Kif26b
  • GenBank ID
    NP_001155137.1
  • Entrez Gene
    Kif26b (a.k.a. 4832420M10, BC056349, D230039L06Rik)
  • Promoter EF1A
  • Tag / Fusion Protein
    • GFP

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX307 GFP-Kif26b-C was a gift from Henry Ho (Addgene plasmid # 118483 ; http://n2t.net/addgene:118483 ; RRID:Addgene_118483)

  • For your References section:

    Identification of a WNT5A-Responsive Degradation Domain in the Kinesin Superfamily Protein KIF26B. Karuna EP, Choi SS, Scales MK, Hum J, Cohen M, Fierro FA, Ho HH. Genes (Basel). 2018 Apr 5;9(4). pii: genes9040196. doi: 10.3390/genes9040196. 10.3390/genes9040196 PubMed 29621187
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