pFA6a-ytdTomato-CaURA3
(Plasmid #118461)

• Purpose: Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection
• Depositing Lab: Bas Teusink
• Publication: Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.

Backbone

• Vector backbone: pFA6a
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ytdTomato
• Species: Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pFA6a-link-ytdTomato-CaURA3 .ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note the absence of a linker region at the 5’ end of the FP. Using a FW primer upstream of the FP will introduce a stopcodon. Please use FW primers annealing on the FP itself.