pCAG-SpCas9-2A-GFP-noITR
(Plasmid #118415)

• Purpose: Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression
• Depositing Lab: Mark Denham
• Publication: Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. doi: 10.3389/fcell.2018.00054. eCollection 2018.

Backbone

• Vector backbone: pSpCas9(BB)-2A-GFP (PX458)
• Modifications to backbone: Plasmid ITR regions were removed by restriction digestion with NotI and SbfI to remove a 145 bp fragment. A fragment containing a BamHI site with two sticky ends compatible with the NotI and SbfI was cloned in. doi: 10.3389/fcell.2018.00054
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry