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Addgene

pBHDuet50K
(Plasmid #118399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MvuTFIIB mini intein
  • Mutation
    incorporation of oligonucleotide during cloning

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBHDuet50K was a gift from Hideo Iwai (Addgene plasmid # 118399 ; http://n2t.net/addgene:118399 ; RRID:Addgene_118399)

  • For your References section:

    Structural Basis for the Persistence of Homing Endonucleases in Transcription Factor IIB Inteins. Iwai H, Mikula KM, Oeemig JS, Zhou D, Li M, Wlodawer A. J Mol Biol. 2017 Dec 8;429(24):3942-3956. doi: 10.1016/j.jmb.2017.10.016. Epub 2017 Oct 18. 10.1016/j.jmb.2017.10.016 PubMed 29055778
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