pBHDuet173
(Plasmid
#118398)
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Purposeanalysis of cis-splicing by mini inteins
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSFDuet
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMvuTFIIB mini intein
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBHDuet173 was a gift from Hideo Iwai (Addgene plasmid # 118398 ; http://n2t.net/addgene:118398 ; RRID:Addgene_118398) -
For your References section:
Structural Basis for the Persistence of Homing Endonucleases in Transcription Factor IIB Inteins. Iwai H, Mikula KM, Oeemig JS, Zhou D, Li M, Wlodawer A. J Mol Biol. 2017 Dec 8;429(24):3942-3956. doi: 10.1016/j.jmb.2017.10.016. Epub 2017 Oct 18. 10.1016/j.jmb.2017.10.016 PubMed 29055778