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Purpose(Empty Backbone) Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118375 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerThermo Fisher Scientific
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Vector typeMammalian Expression ; Destination
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Tag
/ Fusion Protein
- HA-BirA* (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- HA-BirA* (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-BirA*-pDEST-N-pcDNA5/FRT/TO was a gift from Maria Vartiainen (Addgene plasmid # 118375 ; http://n2t.net/addgene:118375 ; RRID:Addgene_118375) -
For your References section:
Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing. Viita T, Kyheroinen S, Prajapati B, Virtanen J, Frilander MJ, Varjosalo M, Vartiainen MK. J Cell Sci. 2019 Apr 17;132(8). pii: jcs.226852. doi: 10.1242/jcs.226852. 10.1242/jcs.226852 PubMed 30890647
