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Addgene

hHSF1 S307A
(Plasmid #118364)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118364 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/myc-His(-) A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5522
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hHSF1
  • Species
    H. sapiens (human)
  • Mutation
    S307A
  • Entrez Gene
    HSF1 (a.k.a. HSTF1)
  • Tag / Fusion Protein
    • myc and 6xHIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hHSF1 S307A was a gift from Lea Sistonen (Addgene plasmid # 118364 ; http://n2t.net/addgene:118364 ; RRID:Addgene_118364)

  • For your References section:

    Phosphorylation of serine 303 is a prerequisite for the stress-inducible SUMO modification of heat shock factor 1. Hietakangas V, Ahlskog JK, Jakobsson AM, Hellesuo M, Sahlberg NM, Holmberg CI, Mikhailov A, Palvimo JJ, Pirkkala L, Sistonen L. Mol Cell Biol. 2003 Apr . 23(8):2953-68. 10.1128/MCB.23.8.2953-2968.2003 PubMed 12665592
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