hSUMO1-HIS
(Plasmid #118361)

• Purpose: This plasmid expresses the full length human SUMO1.
• Depositing Lab: Lea Sistonen
• Publication: Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Backbone

• Vector backbone: pSG5
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4076
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hSUMO1
• Species: H. sapiens (human)
• Mutation: Please see Depositor Comments
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Tag / Fusion Protein:
  • HIS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results found S2A within the SUMO1 translation compared to the closest NCBI reference (NP_003343.1). The depositing laboratory affirms that to their knowledge, this mutation does not change protein function