hSUMO1 GFP
(Plasmid #118360)

• Purpose: This plasmid expresses the full length human SUMO1.
• Depositing Lab: Lea Sistonen
• Publication: Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Backbone

• Vector backbone: pEGFP-C2
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 4735
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hSUMO1
• Species: H. sapiens (human)
• Mutation: WT
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry