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Addgene

Gal4-HSF4b WT
(Plasmid #118358)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118358 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSG424
  • Backbone manufacturer
    Sadowski and Ptashne
  • Backbone size w/o insert (bp) 3396
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hHSF4b
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Entrez Gene
    HSF4 (a.k.a. CTM, CTRCT5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gal4-HSF4b WT was a gift from Lea Sistonen (Addgene plasmid # 118358 ; http://n2t.net/addgene:118358 ; RRID:Addgene_118358)
  • For your References section:

    PDSM, a motif for phosphorylation-dependent SUMO modification. Hietakangas V, Anckar J, Blomster HA, Fujimoto M, Palvimo JJ, Nakai A, Sistonen L. Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):45-50. doi: 10.1073/pnas.0503698102. Epub 2005 Dec 21. 10.1073/pnas.0503698102 PubMed 16371476