hHSF4b K294R
(Plasmid #118357)

• Purpose: This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.
• Depositing Lab: Lea Sistonen
• Publication: Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):45-50. doi: 10.1073/pnas.0503698102. Epub 2005 Dec 21.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hHSF4b
• Species: H. sapiens (human)
• Mutation: K294R
• Entrez Gene: HSF4 (a.k.a. CTM, CTRCT5)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a longer protein (isoform b). This variant, alternatively referred to as HSF4b, functions as a transcriptional activator of the constitutive expression of heat shock genes.