hMEF2A K403R
(Plasmid #118355)

• Purpose: This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.
• Depositing Lab: Lea Sistonen
• Publication: Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):45-50. doi: 10.1073/pnas.0503698102. Epub 2005 Dec 21.

Backbone

• Vector backbone: pcDNA3.1/myc-His(-) A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5522
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hMEF2A
• Species: H. sapiens (human)
• Mutation: K403R
• Entrez Gene: MEF2A (a.k.a. ADCAD1, RSRFC4, RSRFC9, mef2)
• Tag / Fusion Protein:
  • myc and 6xHIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry