hGATA-1 K137R
(Plasmid #118353)

• Purpose: This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.
• Depositing Lab: Lea Sistonen
• Publication: Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):45-50. doi: 10.1073/pnas.0503698102. Epub 2005 Dec 21.

Backbone

• Vector backbone: pcDNA3.1/myc-His(-) A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5522
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hGATA-1
• Species: H. sapiens (human)
• Mutation: K137R
• Entrez Gene: GATA1 (a.k.a. ERYF1, GATA-1, GF-1, GF1, HAEADA, NF-E1, NFE1, XLANP, XLTDA, XLTT)
• Tag / Fusion Protein:
  • myc and 6xHIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry