hHSF1 K80Q
(Plasmid #118351)

• Purpose: Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.
• Depositing Lab: Lea Sistonen
• Publication: Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Backbone

• Vector backbone: pcDNA3.1/myc-His(-) A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5522
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hHSF1
• Species: H. sapiens (human)
• Mutation: K80Q
• Entrez Gene: HSF1 (a.k.a. HSTF1)
• Tag / Fusion Protein:
  • myc and 6xHIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry