hHSF1 RNAi resistant
(Plasmid #118347)

• Purpose: This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).
• Depositing Lab: Lea Sistonen
• Publication: Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86. doi: 10.1074/jbc.M607556200. Epub 2007 Jan 9.

Backbone

• Vector backbone: pcDNA3.1/myc-His(-) A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5522
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hHSF1
• Species: H. sapiens (human)
• Mutation: RNAi resistance mutations (does not change amino acid sequence)
• Entrez Gene: HSF1 (a.k.a. HSTF1)
• Tag / Fusion Protein:
  • myc and 6xHIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry