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Addgene

pSUPER-hHSF1 shRNA
(Plasmid #118345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118345 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPER
  • Backbone manufacturer
    Oligoengine
  • Backbone size w/o insert (bp) 3176
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hHSF1 shRNA
  • gRNA/shRNA sequence
    GCT CAT TCA GTT CCT GAT C
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Entrez Gene
    HSF1 (a.k.a. HSTF1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER-hHSF1 shRNA was a gift from Lea Sistonen (Addgene plasmid # 118345 ; http://n2t.net/addgene:118345 ; RRID:Addgene_118345)

  • For your References section:

    Heat shock factor 2 (HSF2) contributes to inducible expression of hsp genes through interplay with HSF1. Ostling P, Bjork JK, Roos-Mattjus P, Mezger V, Sistonen L. J Biol Chem. 2007 Mar 9;282(10):7077-86. doi: 10.1074/jbc.M607556200. Epub 2007 Jan 9. 10.1074/jbc.M607556200 PubMed 17213196
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