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Addgene

Flag-Craf SSDD
(Plasmid #118337)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118337 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Craf SSDD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1947
  • Mutation
    SSDD
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

site directed mutagenesis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-Craf SSDD was a gift from Philip Stork (Addgene plasmid # 118337 ; http://n2t.net/addgene:118337 ; RRID:Addgene_118337)

  • For your References section:

    Phosphorylation of the C-Raf N-region promotes Raf dimerization. Takahashi M, Li Y, Dillon TJ, Kariya Y, Stork PJS. Mol Cell Biol. 2017 Jul 10. pii: MCB.00132-17. doi: 10.1128/MCB.00132-17. 10.1128/MCB.00132-17 PubMed 28694330
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