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Flag-KSR R615H
(Plasmid #118332)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118332 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KSR R615H
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2622
  • Mutation
    R615H
  • Entrez Gene
    Ksr1 (a.k.a. B-KSR1, D11Bhm183e, D11Bhm184e, Ksr, RSU2, hb, mKSR1)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    human KSR was obtained from Andrey Shaw (Washington University).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

KSR construct was cloned in pcDNA3 containing Flag epitope with EcoRI and XbaI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-KSR R615H was a gift from Philip Stork (Addgene plasmid # 118332 ; http://n2t.net/addgene:118332 ; RRID:Addgene_118332)

  • For your References section:

    Phosphorylation of Rap1 by cAMP-dependent Protein Kinase (PKA) Creates a Binding Site for KSR to Sustain ERK Activation by cAMP. Takahashi M, Li Y, Dillon TJ, Stork PJ. J Biol Chem. 2017 Jan 27;292(4):1449-1461. doi: 10.1074/jbc.M116.768986. Epub 2016 Dec 21. 10.1074/jbc.M116.768986 PubMed 28003362
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