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GFP-Rap1b S179AS180A
(Plasmid #118327)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118327 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peGFP-C1
  • Backbone manufacturer
    clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rap1b S179AS180A
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    555
  • Mutation
    S179AS180A
  • Entrez Gene
    RAP1B
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site ApaI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Rap1b S179AS180A was a gift from Philip Stork (Addgene plasmid # 118327 ; http://n2t.net/addgene:118327 ; RRID:Addgene_118327)

  • For your References section:

    Protein kinase A-dependent phosphorylation of Rap1 regulates its membrane localization and cell migration. Takahashi M, Dillon TJ, Liu C, Kariya Y, Wang Z, Stork PJ. J Biol Chem. 2013 Sep 27;288(39):27712-23. doi: 10.1074/jbc.M113.466904. Epub 2013 Aug 14. 10.1074/jbc.M113.466904 PubMed 23946483
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