Skip to main content
Addgene

Flag-Rap1b
(Plasmid #118325)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118325 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rap1b
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    555
  • Entrez Gene
    RAP1B
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-Rap1b was a gift from Philip Stork (Addgene plasmid # 118325 ; http://n2t.net/addgene:118325 ; RRID:Addgene_118325)

  • For your References section:

    Protein kinase A-dependent phosphorylation of Rap1 regulates its membrane localization and cell migration. Takahashi M, Dillon TJ, Liu C, Kariya Y, Wang Z, Stork PJ. J Biol Chem. 2013 Sep 27;288(39):27712-23. doi: 10.1074/jbc.M113.466904. Epub 2013 Aug 14. 10.1074/jbc.M113.466904 PubMed 23946483
Related items:
Commonly requested with: