GFP-RanV19 E1RA2
(Plasmid #118317)

• Purpose: Expression of human Ran
• Depositing Lab: Philip Stork
• Publication: Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69. doi: 10.1128/MCB.00242-10. Epub 2010 Jun 14.

Backbone

• Vector backbone: peGFP-C1
• Backbone manufacturer: clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: RanV19
• Species: H. sapiens (human)
• Insert Size (bp): 3350
• Mutation: in-frame insertion of RanV19 into GFPEpac1RA2
• Entrez Gene: RAN (a.k.a. ARA24, Gsp1, TC4)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Gene/Insert 2

• Gene/Insert name: EPac1RA2
• Alt name: Rapgef4
• Species: H. sapiens (human)
• Mutation: see comments
• Entrez Gene: RAPGEF3 (a.k.a. CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910)
• Entrez Gene: RAPGEF4 (a.k.a. CAMP-GEFII, CGEF2, EPAC, EPAC 2, EPAC2, Nbla00496)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RanV19 was subcloned into GFP-Epac1RA2 with HindIII and EcoRI

RA2 of Epac2 was inserted between aa 423 and 586 of Epac1.