GFP-RalGDS
(Plasmid #118315)

• Purpose: Expression of human RalGDS
• Depositing Lab: Philip Stork
• Publication: Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69. doi: 10.1128/MCB.00242-10. Epub 2010 Jun 14.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RalGDS
• Species: H. sapiens (human)
• Insert Size (bp): 291
• Mutation: fragment of human RalGDS 203aa
• Entrez Gene: RALGDS (a.k.a. RGDS, RGF, RalGEF)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: RalGDS was obtained from Johannes Bos (Utrecht University)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry