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Addgene

GFP-Epac2
(Plasmid #118314)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118314 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peGFP-C1
  • Backbone manufacturer
    clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Epac2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2982
  • Entrez Gene
    Rapgef4 (a.k.a. 1300003D15Rik, 5730402K07Rik, 6330581N18Rik, EPAC 2, Epac-2, Epac2, mKIAA4040)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (unknown if destroyed)
  • 3′ cloning site blunted NotI/SmaI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    HA-mouse Epac2 was obtained from Lawrence Quilliam (Indiana University)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Epac2 was a gift from Philip Stork (Addgene plasmid # 118314 ; http://n2t.net/addgene:118314 ; RRID:Addgene_118314)

  • For your References section:

    Ras is required for the cyclic AMP-dependent activation of Rap1 via Epac2. Liu C, Takahashi M, Li Y, Song S, Dillon TJ, Shinde U, Stork PJ. Mol Cell Biol. 2008 Dec;28(23):7109-25. doi: 10.1128/MCB.01060-08. Epub 2008 Sep 29. 10.1128/MCB.01060-08 PubMed 18824540
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