GFP-Epac1 CAAX
(Plasmid #118312)

• Purpose: Expression of human Epac1
• Depositing Lab: Philip Stork
• Publication: Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. doi: 10.1128/MCB.26.6.2130-2145.2006.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Epac1 CAAX
• Species: H. sapiens (human)
• Insert Size (bp): 2646
• Mutation: C-terminal 20aa of KiRas
• Entrez Gene: RAPGEF3 (a.k.a. CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: BGH

Resource Information

• A portion of this plasmid was derived from a plasmid made by: human Epac1 was obtained from Johannes Bos (Utrecht University)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry