Flag-Src
(Plasmid #118308)

• Purpose: Expression of mouse Src
• Depositing Lab: Philip Stork
• Publication: Schmitt et al Mol Cell. 2002 Jan;9(1):85-94.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Src
• Species: M. musculus (mouse)
• Insert Size (bp): 1606
• Entrez Gene: Src (a.k.a. pp60c-src)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Src constructs were subcloned into pcDNA3 containing Flag epitope with HindIII and BamHI.