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Addgene

Flag-Fos S362D
(Plasmid #118303)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C-Fos S362D
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1143
  • Mutation
    S362D
  • Entrez Gene
    Fos (a.k.a. D12Rfj1, c-fos, cFos)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

site directed mutagenesis. Fos constructs were subcloned into pcDNA3 containing Flag epitope with EcoRI and Xba.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-Fos S362D was a gift from Philip Stork (Addgene plasmid # 118303 ; http://n2t.net/addgene:118303 ; RRID:Addgene_118303)

  • For your References section:

    Sustained activation of extracellular signal-regulated kinase by nerve growth factor regulates c-fos protein stabilization and transactivation in PC12 cells. Pellegrino MJ, Stork PJ. J Neurochem. 2006 Dec;99(6):1480-93. doi: 10.1111/j.1471-4159.2006.04250.x. 10.1111/j.1471-4159.2006.04250.x PubMed 17223854
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