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Addgene

GFP-PKI WT
(Plasmid #118300)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118300 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peGFP-C1
  • Backbone manufacturer
    clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKI wt
  • Alt name
    protein kinase inhibitor peptide
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    231
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PKIa was obtained from Richard Maurer (Oregon Health and Science University).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PKI constructs were subcloned into pEGFP containing Flag epitope with EcoRI and XbaI (see map).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-PKI WT was a gift from Philip Stork (Addgene plasmid # 118300 ; http://n2t.net/addgene:118300 ; RRID:Addgene_118300)

  • For your References section:

    Hormonal control of insulin-like growth factor I gene transcription in human osteoblasts: dual actions of cAMP-dependent protein kinase on CCAAT/enhancer-binding protein delta. Billiard J, Grewal SS, Lukaesko L, Stork PJ, Rotwein P. J Biol Chem. 2001 Aug 17;276(33):31238-46. doi: 10.1074/jbc.M103634200. Epub 2001 Jun 4. 10.1074/jbc.M103634200 PubMed 11390399
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